|
KMID : 1007520210300070959
|
|
Food Science and Biotechnology
2021 Volume.30 No. 7 p.959 ~ p.969
|
|
|
Inulinase from Rhodotorula mucilaginosa: immobilization and application in the production of fructooligosaccharides
|
|
de Araujo Ribeiro Geise Camila
Fernandes Pedro
Silva Dayse Alessandra Almeida
Brandao Hugo Neves
de Assis Sandra Aparecida
|
|
|
Abstract
|
|
|
|
The crude extract containing inulinase from Rhodotorula mucilaginosa was obtained by submerged fermentation. Inulinase was immobilized on chicken eggshell by physical adsorption and covalent crosslinking, using glutaraldehyde as a crosslinking reagent, and Celite by adsorption. Fructooligosaccharides production was performed using immobilized inulinase (5%, w/v) and inulin substrate solution under experimental conditions evaluated through Doehlert experimental design. The production of inulinase was optimized for concentrations of D-glucose and yeast extract at 12.5 and 0.5 g/L, respectively, resulting in an optimal activity of 0.62 U. The optimal pH and temperature for enzyme activity were 8.0 and 75 °C, respectively, leading to an optimal activity of 3.54 U. The highest immobilization efficiency (46.27%) was obtained upon immobilization on Celite. Immobilization by adsorption to eggshell allowed for specific activity of 4.15 U/g, and adsorption to Celite resulted in specific activity of 3.70 U/g. The highest titer in fructooligosaccharides was obtained with an initial inulin concentration of 250 g/L (25%, w/v), and a reaction time of 16 h. Hence, immobilized inulinase proved to be a promising catalyst for fructooligosaccharides production since the formulation is performed through a simple, low-cost, and large-scale applicable methodology.
|
|
|
KEYWORD
|
|
|
Hydrolase, Immobilization, Inulin, Optimization, Yeast
|
|
|
FullTexts / Linksout information
|
|
|
|
|
|
Listed journal information
|
|
 
|
|